导读 您好,今天小编胡舒来为大家解答以上的问题。tm值的概念,tm值相信很多小伙伴还不知道,现在让我们一起来看看吧!1、博凌科为-为你解答:先看...

您好,今天小编胡舒来为大家解答以上的问题。tm值的概念,tm值相信很多小伙伴还不知道,现在让我们一起来看看吧!

1、博凌科为-为你解答:先看一下Tm的定义:Tm = Temperature at which 50% of a given oligonucleotide is hybridized to its complementary strand. In the absence of destabilizing agents, like formamide or urea, Tm will depend on 3 major parameters: The sequence: a GC-rich sequence has a higher melting temperature. The strand concentration: high oligonucleotide concentrations favor hybrid formation, which results in a higher melting temperature. The salt concentration: high ionic strength results in a higher Tm as cations stabilize the DNA duplexes.引物设计软件都可以给出Tm,引物长度,碱基组成,引物使用缓冲的离子强度有关。

2、长度为25mer以下的引物,Tm计算公式为:Tm = 4℃(G + C)+ 2℃(A + T)对于更长的寡聚核苷酸,Tm计算公式为:Tm = 81.5 + 16.6 x Log10[Na+] + 0.41 (%GC) 600/size公式中,Size = 引物长度。

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